Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

iPS cells

NA

NA

Sample

source_name

caMKL1 blocked cells at day 25

cell type

iPS-like cells

passages

Day 25

antibody

Millipore, 07-473

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNA was extracted with Trizol; ChIP DNA was extracted with Qiagen Gel Extraction kit; ATAC-seq DNA was extracted with Qiagen MinElute PCR purification kit RNA-seq libraries were prepared with TruSeq Stranded mRNA Library Prep Kit (Illumina, RS-122-2101); ChIP DNA libraries were constructed with ThruPLEX® DNA-seq Kit (Rubicon Genomics), ATAC-seq libraries were prepared with Illumina Nextera DNA Library Prep Kit (15028212).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

6771728

Reads aligned (%)

98.8

Duplicates removed (%)

7.2

Number of peaks

16089 (qval < 1E-05)

mm9

Number of total reads

6771728

Reads aligned (%)

98.6

Duplicates removed (%)

7.4

Number of peaks

16135 (qval < 1E-05)