Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mouse embryonic stem cells
cell line
E14 cell line
cell type
embryonic stem cells
ip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysed using SDS-based lysis buffer and sonicated, protein-DNA complexes were then isolated with antibody. Immunoprecipitated protein-DNA complex were ligated with illumina-compatible adaptors, amplified by PCR and sequenced.

Sequencing Platform

instrument_model
Illumina MiSeq

mm10

Number of total reads
10902590
Reads aligned (%)
97.6
Duplicates removed (%)
11.8
Number of peaks
400 (qval < 1E-05)

mm9

Number of total reads
10902590
Reads aligned (%)
97.4
Duplicates removed (%)
12.0
Number of peaks
394 (qval < 1E-05)

Base call quality data from DBCLS SRA