SRX4734090
GSM3399684: RNAPolII ChIP rep2 input; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mouse embryonic stem cells
cell line
E14 cell line
cell type
embryonic stem cells
ip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysed using SDS-based lysis buffer and sonicated, protein-DNA complexes were then isolated with antibody. Immunoprecipitated protein-DNA complex were ligated with illumina-compatible adaptors, amplified by PCR and sequenced.

Sequencing Platform

instrument_model
Illumina MiSeq

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
8615680
Reads aligned (%)
98.0
Duplicates removed (%)
10.4
Number of peaks
374 (qval < 1E-05)

mm9

Number of total reads
8615680
Reads aligned (%)
97.8
Duplicates removed (%)
10.7
Number of peaks
349 (qval < 1E-05)

Base call quality data from DBCLS SRA