SRX4734087
GSM3399681: RNAPolII ChIP rep3; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Mouse embryonic stem cells
cell line
E14 cell line
cell type
embryonic stem cells
ip antibody
RNA polymerase II clone (Covance MMS126R-500)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were lysed using SDS-based lysis buffer and sonicated, protein-DNA complexes were then isolated with antibody. Immunoprecipitated protein-DNA complex were ligated with illumina-compatible adaptors, amplified by PCR and sequenced.

Sequencing Platform

instrument_model
Illumina MiSeq

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
11398399
Reads aligned (%)
97.3
Duplicates removed (%)
10.7
Number of peaks
5921 (qval < 1E-05)

mm9

Number of total reads
11398399
Reads aligned (%)
97.1
Duplicates removed (%)
10.9
Number of peaks
5930 (qval < 1E-05)

Base call quality data from DBCLS SRA