GSM1331245: RD human IgG ChIP-Seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Muscle
Cell type
RD
Primary Tissue
Skelatal Muscle
Tissue Diagnosis
Rhabdomyosarcoma
Attributes by original data submitter
Sample
source_name
RD human cell line
cell line
RD
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RD cells or cells derived from YAP-driven tumors under Myf5-Cre or Pax7-CreER cells were cross-linked in 1% formaldehyde for 10 minutes at room temperature after which the reaction was stopped by addition of 0.125M glycine. Cells were lysed and harvested in ChIP buffer Chromatin was disrupted by sonication using a Diagenode Bioruptor sonicator UCD-200 to obatin fragments of average 200-500 b in size. Suitable amounts of chromatin were incubated with specific antibodies overnight. Immunoprecipitated complexes were recovered on Protein-A/G agarose beads (Pierce) or Protein G Dynabeads (Invitrogen). After extensive washes, DNA was recovered by reverse crosslinking and purification using QIAquick PCR purification kit (Qiagen) ChIP-Seq libraries were built using the NEBNext Ultra DNA Library Prep Kit for Illumina (NEB, E7370) according to manufacturer's recommendation. Libraries for each celle lines were barcoded using NEBNext multiplex oligos for Illumina (NEB, E7335) and run on a single lane of HiSeq2000 (Illumina)