Antigen

Antigen Class

TFs and others

Antigen

TfIIB

Cell type

Cell type Class

Embryo

Cell type

14-17h embryos

NA

NA

Sample

source_name

14-17hrs OregonR embryo

tissue

embryos

chip antibody

anti-TFIIB rabbit polyclonal, custom

developmental stage

14-17h

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq was performed according to He et al. (2011) and He et al. (2015) with whole cell extract (WCE) derived from 100 mg embryos per ChIP ChIP-seq libraries were constructed following He et al. (2011)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

26232448

Reads aligned (%)

96.3

Duplicates removed (%)

25.4

Number of peaks

6138 (qval < 1E-05)

dm3

Number of total reads

26232448

Reads aligned (%)

96.5

Duplicates removed (%)

23.5

Number of peaks

5431 (qval < 1E-05)