Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AGO2

Cell type

Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular

Attributes by original data submitter

Sample

source_name
HepG2
biomaterial_provider
ATCC (HB-8065)
cell line
HepG2
cell type
Hepatocellular carcinoma
chip antibody
AGO2 (Abnova Cat# H00027161-M01)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Nuclei were isolated and sonicated and lysates were cleared-up and applied for purification of RBP-DNA complexes with antibodies. Libraries were constructed according to the instruction of the Illumina Preparing Samples for ChIP Sequencing.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
68851365
Reads aligned (%)
55.8
Duplicates removed (%)
31.2
Number of peaks
26617 (qval < 1E-05)

hg38

Number of total reads
68851365
Reads aligned (%)
56.7
Duplicates removed (%)
30.6
Number of peaks
26665 (qval < 1E-05)

Base call quality data from DBCLS SRA