Antigen

Antigen Class

Histone

Antigen

H3K9me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

Rif1 knockdown ESCs

cell type

mouse ESCs

genotype

Rif1 knowckdown

chip antibody

Abcam ab8898

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed and chromatin was extracted after sonication for ChIP experiments using H3K9me3 antibody. Genomic DNA was used as input DNA. Libraries were prepared according to Illumina's standard protocol.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

28561565

Reads aligned (%)

95.0

Duplicates removed (%)

37.5

Number of peaks

6832 (qval < 1E-05)

mm9

Number of total reads

28561565

Reads aligned (%)

94.2

Duplicates removed (%)

37.3

Number of peaks

7002 (qval < 1E-05)