Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Epidermis

Cell type

Dermal fibroblast

NA

NA

Sample

source_name

Skin fibroblast cells

biomaterial_provider

AG16409

cell type

Primary Dermal Fibroblast

biopsy site

skin, unspecified

Sequenced DNA Library

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

library_construction_protocol

Primary fibroblast and lymphoblastoid (LCLs) cell were grown in culture then pelleted before immediately proceeding to DNase-seq library preparations. All DNase-Seq libraries were prepared according to (Song L, et al. Genome Res. 2011; PMID 21750106). Briefly, cells were lysed and incubated at 37¡C for various times (5 min to 1 hour) and optimal DNase digestion was confirmed by pulsed field gel electrophoresis (Song L and Crawford GE. Cold Spring Harbor protocols 2010(2):pdb prot5384). DNase-Seq libraries were prepared according to (Song L, et al. Genome Res. 2011; PMID 21750106).

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg38

Number of total reads

50938241

Reads aligned (%)

1.9

Duplicates removed (%)

30.2

Number of peaks

8 (qval < 1E-05)

hg19

Number of total reads

50938241

Reads aligned (%)

1.9

Duplicates removed (%)

31.0

Number of peaks

11 (qval < 1E-05)