GSM1325254: H3K4me3 Ctrl ChIP-Seq; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H4K4me3
Cell type
Cell type Class
Breast
Cell type
SK-BR-3
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
SKBr3
cell line
SKBr3
cell type
breast cancer epithelial cells
chip antibody
H4K4me3 (Abcam, ab1012)
treatment
control
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was isolated from the nuclei of formaldehyde cross-linked cells, sonicated and immunoprecipated with anti-H3K4me3 antibody. ChIP-Seq libraries were constructed from 10ng immunoprecipitated or original input chromatin using the ChIP-Seq Sample Prep Kit from Illumina, following the manufacturer's instructions.