Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
BEko
NA
NA

Attributes by original data submitter

Sample

source_name
Beko pre-T cell line
cell line
Beko pre-T
chip antibody
diagenode, pAb-174-050
treatment
24 hours with 0.01 mg/ml apicidin.

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP was done essentially as previously described (Giaimo et al. Jove 2017, doi: 10.3791/55907) Lybrary-prep kit: Diagenode MicropPlex Library Preparation kit v2 (C05010012)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
22297624
Reads aligned (%)
97.2
Duplicates removed (%)
5.8
Number of peaks
22489 (qval < 1E-05)

mm9

Number of total reads
22297624
Reads aligned (%)
97.1
Duplicates removed (%)
5.9
Number of peaks
22446 (qval < 1E-05)

Base call quality data from DBCLS SRA