GSM3382306: GSC23 H3K27AC INPUT; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Neural
Cell type
Glioblastoma stem cells
NA
NA
Attributes by original data submitter
Sample
source_name
glioblastoma stem cells
cell line
3752
input
NONE
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
5 million cells were crosslinked in 1% formaldehyde for 10 minutes, lysed, sonicated to ~500bp, and immunoprecipated using H3K27ac antibody (Active Motif 39133). In the case of tissue, 5-10mg of tissue was pulvarized in liquid nitrogen before cross-linking in the same manner as cell protocols (above) ChIP DNA was purified using the Qiagen PCR purification kit. Libraries were prepared according to manufacturers instructions using the Rubicon Thruplex DNA-seq kit Libraries were constructed using the DNA-seq kit from Rubicon Genomics and quantified using Picogreen