Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Liver

strain

C57BL/6

tissue

Liver

genotype

wild type

molecule enrichment protocol

GR ChIP-seq, MA1-510 (ThermoFischer), PA1-511a (ThermoFischer), SC-1003 (Santa Cruz)

treatment

Night restricted feeding, pre-prandial injection with vehicle

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked sonicated chromatin was IP'ed using antibodies against H3K27Ac, GR or FOXO1. DNA was extracted by phenol/choloroform. ChIP-seq and DHS-seq libraries were constructed using the NEBNext Library prep kit according to manufacturer´s (NEB) instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

13800361

Reads aligned (%)

96.4

Duplicates removed (%)

21.6

Number of peaks

2214 (qval < 1E-05)

mm9

Number of total reads

13800361

Reads aligned (%)

96.2

Duplicates removed (%)

21.7

Number of peaks

2212 (qval < 1E-05)