Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Liver

strain

C57BL/6

tissue

Liver

genotype

wild type

molecule enrichment protocol

FoxO1 ChIP-seq, sc-11350 (Santa Cruz)

treatment

Night restricted feeding

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked sonicated chromatin was IP'ed using antibodies against H3K27Ac, GR or FOXO1. DNA was extracted by phenol/choloroform. ChIP-seq and DHS-seq libraries were constructed using the NEBNext Library prep kit according to manufacturer´s (NEB) instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 1500

mm10

Number of total reads

22853283

Reads aligned (%)

89.2

Duplicates removed (%)

35.6

Number of peaks

1516 (qval < 1E-05)

mm9

Number of total reads

22853283

Reads aligned (%)

89.1

Duplicates removed (%)

35.8

Number of peaks

1536 (qval < 1E-05)