Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

ChIP-seq

agent

DRBI

chip antibody

sc-899X, RNA polymerase II (N-20) X (Santa Cruz Biotechnology)

cell line

293

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using Illumina's TruSeq ChIP library Preparation Kit according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

40136910

Reads aligned (%)

94.7

Duplicates removed (%)

21.0

Number of peaks

14545 (qval < 1E-05)

hg19

Number of total reads

40136910

Reads aligned (%)

94.1

Duplicates removed (%)

21.2

Number of peaks

14256 (qval < 1E-05)