Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

Macrophages

MeSH Description

The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

Sample

source_name

Primary bone marrow-derived macrophages (BMDM)

strain

C57BL/6

cell type

BMDM cells (7th day of differentiation)

treatment

LPS (10 ng/ml) for 30'

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

ATAC-seq libraries were prepared for HiSeq 4000 sequencing following standard protocols.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

30780603

Reads aligned (%)

80.3

Duplicates removed (%)

58.4

Number of peaks

71930 (qval < 1E-05)

mm9

Number of total reads

30780603

Reads aligned (%)

80.3

Duplicates removed (%)

58.5

Number of peaks

71915 (qval < 1E-05)