GSM3377528: THP-1 8h after 1,25D treatment, 3rd replicate H3K4me3; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Blood
Cell type
THP-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous
Attributes by original data submitter
Sample
source_name
Culture
cell line
THP-1
treatment protocol
8h with 1,25(OH)2D3
chip antibody
H3K4me3
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were grown in culture as mentioned above, after which they were crosslinked with formaldehyde, lysed and the DNA was extracted. Then, ChIP was performed using antibody against H3K4me3 (Millipore, cat.# 07-473, lot# 2557645). ChIP DNA was sent to EMBL and the library was prepared there according to their protocol.