Antigen

Antigen Class

TFs and others

Antigen

Tbr1

Cell type

Cell type Class

Neural

Cell type

Cortex

NA

NA

Sample

source_name

P2 Wildtype Cortex_IgG ChIP-seq

strain

CD1

age

P2

tissue

cortex

chip antibody

TBR1 blocking peptide (Abcam, Cat# ab25853)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and TBR1-DNA complexes were isolated with antibody. ChIP-seq libraries were generated using Ovation Ultralow System V2 (NuGEN) following manufacturer's protocol. Libraries were sequenced on Illumina HiSeq 4000

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

14535262

Reads aligned (%)

98.0

Duplicates removed (%)

21.3

Number of peaks

317 (qval < 1E-05)

mm9

Number of total reads

14535262

Reads aligned (%)

97.8

Duplicates removed (%)

21.4

Number of peaks

341 (qval < 1E-05)