Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Blood

Cell type

Hematopoietic Stem Cells

MeSH Description

Progenitor cells from which all blood cells derived. They are found primarily in the bone marrow and also in small numbers in the peripheral blood.

Sample

source_name

Embryonic day 14.5 fetal liver hematopoietic stem cells

strain

B6129SF1

tissue

Fetal liver

age

Embryonic day 14.5

chip antibodies

NA

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

E14.5 fetal liver and adult bone marrow were dissected and HSCs were purified using cell surface marker: Lin-Sca1+cKit+CD135-. ChIP reactions were performed using 50,000 FACS-sorted cells. Iped and Input DNA were purified with MinElute PCR Purification Kit. Libraries were constructed using Rubicom ThruPLEX DNA-Seq Kit. Libraries were size-selected at 100-700 bp by AMPure XP beads. Libraries were quantified with KAPA qPCR and bioanalyzer prior to single-end sequencing.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

29199824

Reads aligned (%)

98.2

Duplicates removed (%)

13.6

Number of peaks

423 (qval < 1E-05)

mm9

Number of total reads

29199824

Reads aligned (%)

97.9

Duplicates removed (%)

13.6

Number of peaks

460 (qval < 1E-05)