Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

hESC derived neural cells

NA

NA

Sample

source_name

human neural progenitor cells

cell line/strain

H1

cell type

human neural progenitor cells with deletion of UTX and JMJD3

genotype

UTX and JMJD3 knockout

antibody

H3K27me3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP-seq, 1×107 cells of each sample were crosslinked in 1% formaldehyde with rotation for 10 minutes at room temperature. And then these crosslinked reactions were stopped by 0.125 M glycine with rotation for 5 minutes at room temperature. and cell sample were sonicated, and then ChIPed with H3K27me3 antibodies. DNA libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

35376160

Reads aligned (%)

79.4

Duplicates removed (%)

6.2

Number of peaks

5044 (qval < 1E-05)

hg19

Number of total reads

35376160

Reads aligned (%)

78.7

Duplicates removed (%)

7.6

Number of peaks

4869 (qval < 1E-05)