GSM3355246: mouse mef control Input ChIP; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast
Attributes by original data submitter
Sample
source_name
mouse_mef_control_Input_ChIP
strain background
C57BL/6
tissue/cell type
embryonic fibroblasts
dnmt3b oe
no
treatment time
n/a
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
DNA was either harvested from mouse tissues, or from cultured MEFs. Flash-frozen mouse tissues and cell pellets were lysed in 300-600µl DNA lysis buffer (10 mM Tris-HCl pD 8.0, 10mM EDTA, 10mM NaCl and 0.5% wt/vol SDS) supplemented with 50 ng/µl Dnase-free Rnase (Roche) and 1 ng/µl proteinase K (NEB) at 55°C overnight. After phenol:chloroform extraction and ethanol precipitation, DNA was resuspended in elution buffer (10 mM Tris-HCl pH 8.0, 1mM EDTA) For RRBS: 100 ng genomic DNA was used to make libraries as previously described (Boyle et al., 2012).