Antigen

Antigen Class

Histone

Antigen

H3K27me3

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

mouse_liver_0-3M_dox_H3K27me3_ChIP

strain background

C57BL/6

tissue/cell type

liver

dnmt3b oe

yes

treatment time

3 months

chip-seq antibody

H3K27me3 (Millipore 07-449)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was either harvested from mouse tissues, or from cultured MEFs. Flash-frozen mouse tissues and cell pellets were lysed in 300-600µl DNA lysis buffer (10 mM Tris-HCl pD 8.0, 10mM EDTA, 10mM NaCl and 0.5% wt/vol SDS) supplemented with 50 ng/µl Dnase-free Rnase (Roche) and 1 ng/µl proteinase K (NEB) at 55°C overnight. After phenol:chloroform extraction and ethanol precipitation, DNA was resuspended in elution buffer (10 mM Tris-HCl pH 8.0, 1mM EDTA) For RRBS: 100 ng genomic DNA was used to make libraries as previously described (Boyle et al., 2012).

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

52618551

Reads aligned (%)

98.1

Duplicates removed (%)

72.7

Number of peaks

474 (qval < 1E-05)

mm9

Number of total reads

52618551

Reads aligned (%)

97.8

Duplicates removed (%)

72.8

Number of peaks

565 (qval < 1E-05)