Antigen

Antigen Class

Histone

Antigen

H4K8ac

Cell type

Cell type Class

Breast

Cell type

MMTV-PyMT

NA

NA

Sample

source_name

Mammary tumor tissue

strain

MMTV-PyVT

tissue

Mammary tumor tissue

treatment

No treatment

antibody

H4K8ac

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were constructed by VATHS Universal DNA Library Prep Kit for Illumina (Vazyme ND604). 

Sequencing Platform

instrument_model

HiSeq X Ten

mm10

Number of total reads

25978858

Reads aligned (%)

79.6

Duplicates removed (%)

8.2

Number of peaks

881 (qval < 1E-05)

mm9

Number of total reads

25978858

Reads aligned (%)

79.5

Duplicates removed (%)

8.1

Number of peaks

839 (qval < 1E-05)