Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

U2OS_siCtr_Pol2_S2P_ChIP

cell line

U-2 OS

sirna

siControl

genotype

control

chip antibody

Anti-RNA polymerase II CTD (Abcam, catalog# ab5095, lot# GR231750-2)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Chromain Immunoprecipitation using anti phosphoserine-2 RNA Polymerase antibodies. Sequencing libraries were prepared using the NEB-next ChIP-seq library Kit following the manual instruction and using indexed primers. The individual libraries were generated in paralel for all ChIPs and input samples. Libraries were pooled and sequenced on an Illumina HiSeq 4000 machine.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

48355158

Reads aligned (%)

97.2

Duplicates removed (%)

17.9

Number of peaks

1692 (qval < 1E-05)

hg19

Number of total reads

48355158

Reads aligned (%)

96.1

Duplicates removed (%)

19.5

Number of peaks

1075 (qval < 1E-05)