Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

liver

strain

C57BL/6

tissue

liver

genotype

Bcl6 fl/fl; Albumin-cre

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked nuclei were sonicated and cleared. Protein-DNA complexes were isolated with antbody against BCL6, PPARa, PPARd, or H3K27ac and then IgG paramagnetic beads. After decrosslinking, DNA was isolated with MinElute columns (Qiagen). Kapa Hyper Prep Kit

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

22034610

Reads aligned (%)

97.6

Duplicates removed (%)

16.4

Number of peaks

245 (qval < 1E-05)

mm9

Number of total reads

22034610

Reads aligned (%)

97.3

Duplicates removed (%)

16.5

Number of peaks

235 (qval < 1E-05)