GSM3346492: H3K27ac SF1 F 1 [E10.5]; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Embryo
Cell type
Gonadal progenitor cells
NA
NA
Attributes by original data submitter
Sample
source_name
FACS-sorted XX gonadal progenitor cells
embryonic stage
E10.5
Sex
male
transgenic line
SF1-eGFP
chip-antibody
H3K27ac Active Motif 39133
protocol
Van Galen et al, 2016
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Embryonic gonads were micro-dissected at according stage, removed from the mesonephros and incubated in 0.05% trypsin for 5 minutes at 37C. Gonads were then mechanically dissasociated in PBS/3% BSA and passed through a filter cap to obtain a single cell suspension. Fluorescently-labeled cells were collected by Fluorescence Activated Cell Sorting (FACS). For ATAC-seq, 60K cells were collected per replicate. For ChIP-seq, 50-100K cells were collected per replicate. ATAC-seq libraries were prepared according to Buenrostro et al.2013. ChIP-seq libraries were prepared according to Van Galen et al., 2016. For ChIP-seq, corresponding H3 ChIP was used as Input.