SRX4557393
GSM3334218: UB H3K4me3 ChIPSeq; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Others
Cell type
Ureteric epithelium
NA
NA

Attributes by original data submitter

Sample

source_name
Ureteric epithelium
tissue
Ureteric epithelium
cell type
Hoxb7-GFP+ ureteric epithelium
strain
(C57BL/6J x CBA/J)F2 origin, maintained with C57BL/6J background
genotype
Hoxb7myr- Venus/+
age
E15.5
chip antibody
anti-H3K4me3 (Abcam, ab8580)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Unfixed cells were lysed and treated with Micrococcal nuclease. Libraries were prepared using ThruPLEX-FD or ThruPLEX DNA-seq kit (Rubicon Genomics)

Sequencing Platform

instrument_model
Illumina HiSeq 3000

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
58681479
Reads aligned (%)
96.9
Duplicates removed (%)
74.4
Number of peaks
25071 (qval < 1E-05)

mm9

Number of total reads
58681479
Reads aligned (%)
96.8
Duplicates removed (%)
74.4
Number of peaks
25170 (qval < 1E-05)

Base call quality data from DBCLS SRA