Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Blood

Cell type

Dendritic Cells

MeSH Description

ANTIGEN-PRESENTING CELLS of dendritic cell morphology found in the LYMPH NODES and other lymphoid tissues.

Sample

source_name

H3K4me3_ChIPseq

strain

CBA/J

Sex

female

tissue

Lung

cell type

CD11c+ dendritic cells

age

6-8 weeks

condition

Naïve

treatment

None

chip antibody

H3K4me3 (Abcam ab8580)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

According to previously published ultra-low input native ChIP-seq protocol (Brind'Amour J, Liu S, Hudson M, Chen C, Karimi MM, Lorincz MC. An ultra-low-input native ChIP-seq protocol for genome wide profiling of rare cell populations. Nature Communications, 2015; 6:6033).

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

42818858

Reads aligned (%)

96.6

Duplicates removed (%)

32.8

Number of peaks

452 (qval < 1E-05)

mm9

Number of total reads

42818858

Reads aligned (%)

96.1

Duplicates removed (%)

32.7

Number of peaks

533 (qval < 1E-05)