GSM3331032: ATACseq subject1 abd rep1; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Adipocyte
Cell type
Preadipocytes
NA
NA
Attributes by original data submitter
Sample
source_name
subject1_abd
individual
subject 1
tissue
adipose
origin
abdominal
cell type
preadipocytes
passage
3
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
After trypsinisation, abdominal and GF preadipocytes nuclei were isolated Nuclei were used for tagmentation using Nextera DNA Library Preparation Kit (Illumina). After tagmentation DNA was purified with MinElute PCR Purification Kit (Qiagen). Tagmented DNA was amplified with Kapa Hifi Hot Start Kit (Kapa Biosystems) using 16 PCR cycles. Amplified libraries were purified with Agencourt AMPure XP (Beckman Coulter)