Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Embryonic fibroblast
Cell type
C3H/10T1/2
NA
NA

Attributes by original data submitter

Sample

source_name
10T1/2_Mut_1
cell line
10T1/2
genotype/variation
IDH2 R172K
treatment
None

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Extraction of material for sequencing was performed according to the Omni-ATAC-seq protocol from Corces et al., as in https://doi.org/10.1038/nmeth.4396 The NEBNext Q5 Hot Start HiFi PCR Master Mix with Nextera PCR primers was used for barcoding and library preparation, and purification was performed with AMPure XP beads.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
53699268
Reads aligned (%)
90.3
Duplicates removed (%)
15.9
Number of peaks
45792 (qval < 1E-05)

mm9

Number of total reads
53699268
Reads aligned (%)
90.1
Duplicates removed (%)
16.0
Number of peaks
45749 (qval < 1E-05)

Base call quality data from DBCLS SRA