Antigen

Antigen Class

Bisulfite-Seq

Antigen

Bisulfite-Seq

Cell type

Cell type Class

Blood

Cell type

Blood Cells

MeSH Description

The cells found in the body fluid circulating throughout the CARDIOVASCULAR SYSTEM.

Sample

source_name

blood (B cell-depleted) from mice with the homozygous chr15: 98,835,228 A>T mutation in KMT2D (Ile5482Asn)

cell type

blood (B cell-depleted)

genotype

HOM

Sequenced DNA Library

library_strategy

Bisulfite-Seq

library_source

GENOMIC

library_selection

RANDOM

library_construction_protocol

Genomic DNA was extracted using the AllPrep DNA/RNA kit (Qiagen, USA) Mouse B cell genomic DNA and B cell-depleted blood DNA was sheared to an average size of 800 bp. 200ng of sheared DNA was used for the bisulphite reaction, library preparation and indexing carried out according to the manufacturer's instructions (CEGX TrueMethyl Seq, UK). Paired-end 150bp sequencing was performed for each library on the Illumina HiSeqX platform using the HiSeq X™ Ten Reagent Kit v2.

Sequencing Platform

instrument_model

HiSeq X Ten

mm10

Number of total reads

165632065

Reads aligned (%)

91.1

Coverage rate (×)

6.7

Number of hyper MRs

240411 (qval < 1E-05)

mm9

Number of total reads

165632065

Reads aligned (%)

91.4

Coverage rate (×)

6.7

Number of hyper MRs

239791 (qval < 1E-05)