GSM3320277: 1002-Plasmablasts-U; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
Plasmablasts
NA
NA
Attributes by original data submitter
Sample
source_name
primary blood
donor
1002
cell type
Plasmablasts
lineage
B
treatment
no_treament
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
ATAC-seq libraries were prepared following the Fast-ATAC protocol (Corces et al., 2016). Briefly, frozen cells were thawed, washed in media, aliquoted and resuspended in Tn5 reaction buffer and incubated at 37℃ for 30 min. Transposed samples were purified using MinElute PCR purification columns according to the manufacturer's protocol (Qiagen, Germany). Purified samples were amplified and indexed using custom Nextera barcoded PCR primers as described in Buenrostro et al (Buenrostro et al., 2013). Amplified libraries were purified using MinElute PCR purification columns followed by gel-based size selection to remove free PCR primers.