Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Pluripotent stem cell

Cell type

P19 derived neural cells

NA

NA

Sample

source_name

day 3 neuronal cells

chip antibody

H3K27ac

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were crosslinked with 5% formaldehyde for 5 mins. Nuclei were extracted, chromatin sonicated, and ChIP was performed using protein G agarose using standard procedures. NEB ultra DNA library prep kit

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

43660702

Reads aligned (%)

93.7

Duplicates removed (%)

6.9

Number of peaks

15038 (qval < 1E-05)

mm9

Number of total reads

43660702

Reads aligned (%)

93.5

Duplicates removed (%)

7.0

Number of peaks

15004 (qval < 1E-05)