Antigen

Antigen Class

Histone

Antigen

H3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

P19

Tissue

Embryo

Disease

Teratocarcinoma; Embryonal Carcinoma

Sample

source_name

undifferentiated P19 EC cells

chip antibody

H3

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were crosslinked with 5% formaldehyde for 5 mins. Nuclei were extracted, chromatin sonicated, and ChIP was performed using protein G agarose using standard procedures. NEB ultra DNA library prep kit

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

mm10

Number of total reads

45978588

Reads aligned (%)

95.5

Duplicates removed (%)

12.8

Number of peaks

962 (qval < 1E-05)

mm9

Number of total reads

45978588

Reads aligned (%)

95.3

Duplicates removed (%)

12.9

Number of peaks

932 (qval < 1E-05)