GSM3314744: Control Input DNA 12h; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
mouse Embryonic Stem Cells (ESCs)
treatment
N2B27 medium containing activin A, bFGF, and knockout serum replacement (KOSR , 1%)
cell line
E14Tg2a
passages
20-35
time point
12h
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA-Seq: Total RNA was extracted with Qiazol lysis reagent (Qiagen) treatment and purified using miRNeasy Kit. ChIP-Seq: Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with an antibody. RNA-Seq libraries were prepared according to the TruSeq Stranded Total RNA Gold Kit (Illumina) using random hexamer priming. ChIP-Seq libraries were prepared from size-selected ChIP DNA according to the standard Illimina library preparation protocol