GSM3314731: RNA Pol II ChIP-Seq 48h; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
mouse Embryonic Stem Cells (ESCs)
treatment
N2B27 medium containing activin A, bFGF, and knockout serum replacement (KOSR , 1%)
cell line
E14Tg2a
passages
20-35
time point
48h
antibody
Anti-RNA Polymerase II 8WG16 (Covance MMS-126R)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA-Seq: Total RNA was extracted with Qiazol lysis reagent (Qiagen) treatment and purified using miRNeasy Kit. ChIP-Seq: Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with an antibody. RNA-Seq libraries were prepared according to the TruSeq Stranded Total RNA Gold Kit (Illumina) using random hexamer priming. ChIP-Seq libraries were prepared from size-selected ChIP DNA according to the standard Illimina library preparation protocol