N2B27 medium containing activin A, bFGF, and knockout serum replacement (KOSR , 1%)
cell line
E14Tg2a
passages
20-35
time point
6h
antibody
Anti-H3K4me3 (Abcam ab8580)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
RNA-Seq: Total RNA was extracted with Qiazol lysis reagent (Qiagen) treatment and purified using miRNeasy Kit. ChIP-Seq: Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with an antibody. RNA-Seq libraries were prepared according to the TruSeq Stranded Total RNA Gold Kit (Illumina) using random hexamer priming. ChIP-Seq libraries were prepared from size-selected ChIP DNA according to the standard Illimina library preparation protocol