Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Myeloid Progenitor Cells
MeSH Description
Stem cells derived from HEMATOPOIETIC STEM CELLS. Derived from these myeloid progenitor cells are the MEGAKARYOCYTES; ERYTHROID CELLS; MYELOID CELLS; and some DENDRITIC CELLS.

Attributes by original data submitter

Sample

source_name
myeloid-progenitor-derived cell line
strain
C57BL/6
genotype
p30/p30
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For RNA-seq QIAGEN RNeasy Plus Kit was used, and for ChIP-seq, lysates were sonicated and histone-DNA complexes were isolated with antibody. Libraries were prepared for sequencing using standard Illumina protocols by sequencing facilities.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
61807038
Reads aligned (%)
97.5
Duplicates removed (%)
12.8
Number of peaks
591 (qval < 1E-05)

mm9

Number of total reads
61807038
Reads aligned (%)
97.3
Duplicates removed (%)
12.7
Number of peaks
678 (qval < 1E-05)

Base call quality data from DBCLS SRA