Antigen

Antigen Class

TFs and others

Antigen

Cebpa

Cell type

Cell type Class

Blood

Cell type

Myeloid Progenitor Cells

MeSH Description

Stem cells derived from HEMATOPOIETIC STEM CELLS. Derived from these myeloid progenitor cells are the MEGAKARYOCYTES; ERYTHROID CELLS; MYELOID CELLS; and some DENDRITIC CELLS.

Sample

source_name

myeloid-progenitor-derived cell line

strain

C57BL/6

genotype

p30/p30

chip antibody

C/EBPα C-18 (Santa Cruz, sc-9314)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For RNA-seq QIAGEN RNeasy Plus Kit was used, and for ChIP-seq, lysates were sonicated and histone-DNA complexes were isolated with antibody. Libraries were prepared for sequencing using standard Illumina protocols by sequencing facilities.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

27406233

Reads aligned (%)

54.9

Duplicates removed (%)

34.9

Number of peaks

21075 (qval < 1E-05)

mm9

Number of total reads

27406233

Reads aligned (%)

54.6

Duplicates removed (%)

34.9

Number of peaks

21090 (qval < 1E-05)