GSM3307219: REH RUNX1 rep1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
RUNX1
Cell type
Cell type Class
Blood
Cell type
REH
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Lymphocytic
Attributes by original data submitter
Sample
source_name
REH_RUNX1
cell line
REH
cell type
Pre-B lymphoblast cells
tissue
Peripheral blood
chip antibody
RUNX1 (ab23980, Abcam)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed in 1% formaldehyde (648336, Polysciences) at room temperature for 10 min and then quenched in 100 mM glycine for 1 min. Cells were washed twice in PBS and lysed 15 min in 50 mM Tris-HCl ph8.1/10 mM EDTA/0.5% Empigen BB/1% SDS. Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were generated using TruSeq® ChIP Library Preparation Kit (Illumina) according to the manufacturer's protocol.