Established from islets of 6 weeks old RIP1-Tag2 transgenic mice
passages
10-15
chip antibody
IgG
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin were sonicated and protein-DNA complexes were isolated with indicated antibody. Starting from 10-20ng of ChIP-DNA, the following library preparation steps are performed for each sample: end-repair, a-tailing, adapter ligation, gel purification and a final PCR enrichment. After quality control of the sequencing library on an Agilent Bioanalyzer and quanrtification of the final library on a Qubit, we proceeded with cluster generation and sequencing.