Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Liver

Cell type

Liver

MeSH Description

A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Sample

source_name

Liver from wild-type fed mouse

strain

C57BL/6J

genotype

Wild-type

treatment

fed

tissue

Liver

chip antibody

Xbp-1s (Santa Cruz,sc7610x,lot#D2910)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and XBP-1-DNA complexes were isolated with antibody. NEBNext® UNEBNext DNA Library Prep Master Mix Set for Illumina (cat# E6040L) was used with ChIP-DNA for the construction of sequencing libraries. ChIP libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

37297454

Reads aligned (%)

80.6

Duplicates removed (%)

32.0

Number of peaks

17014 (qval < 1E-05)

mm9

Number of total reads

37297454

Reads aligned (%)

80.3

Duplicates removed (%)

32.0

Number of peaks

17138 (qval < 1E-05)