anti-RNA polymerase II CTD (phospho S2) antibody, Abcam Cat #ab5095
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to manufacturer's instructions, quantified by Qubit (Invitrogen) and TapeStation (Agilent) and sequenced on an Illumina HiSeq2500 (50 SR lane V4 reagents).