Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
MYC

Cell type

Cell type Class
Neural
Cell type
SH-EP
NA
NA

Attributes by original data submitter

Sample

source_name
Human SH-EP/TET21/N (TET21) neuroblastoma cell line
cell type
neuroblastoma cell line
passages
10-15
chip antibody
rabbit polyclonal anti-MYC antibody (produced in-house)N-262

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were sonicated using a Bioruptor® Pico (Diagenode). ChIP was performed with 2µg of a rabbit polyclonal anti-MYC antibody (prepared in-house) incubated with samples overnight at 4ºC. Samples were subsequently washed and de-crosslinked prior to purification using a Qiaquick PCR purification kit (Qiagen) and library preparation.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
27349984
Reads aligned (%)
81.4
Duplicates removed (%)
17.3
Number of peaks
1052 (qval < 1E-05)

hg38

Number of total reads
27349984
Reads aligned (%)
83.4
Duplicates removed (%)
16.0
Number of peaks
818 (qval < 1E-05)

Base call quality data from DBCLS SRA