Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

HSV1 infected with PAA, 6hpi, pooled replicates

cell line

U2OS

cell line source

Human Osteosarcoma, Female, 15 yr old

infected with

HSV1 strain KOS

time point

6hpi

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

ATAC-seq experiments were performed as previously described (Buenrostro et al., 2013). Libraries were processed using Nextera DNA Librarpy Prep Kit

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

120525253

Reads aligned (%)

81.2

Duplicates removed (%)

4.8

Number of peaks

1152 (qval < 1E-05)

hg19

Number of total reads

120525253

Reads aligned (%)

80.3

Duplicates removed (%)

5.4

Number of peaks

888 (qval < 1E-05)