Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Bone

Cell type

U2OS

Tissue

bone

Lineage

mesoderm

Description

osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

Sample

source_name

HSV1 infected, 4hpi, pooled replicates

cell line

U2OS

cell line source

Human Osteosarcoma, Female, 15 yr old

infected with

HSV1 strain KOS

time point

4hpi

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

ATAC-seq experiments were performed as previously described (Buenrostro et al., 2013). Libraries were processed using Nextera DNA Librarpy Prep Kit

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

147022544

Reads aligned (%)

77.9

Duplicates removed (%)

7.6

Number of peaks

1333 (qval < 1E-05)

hg19

Number of total reads

147022544

Reads aligned (%)

77.2

Duplicates removed (%)

8.2

Number of peaks

1015 (qval < 1E-05)