Antigen

Antigen Class

TFs and others

Antigen

Chd4

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

Embryonic stem cells

tissue

Embryonic stem cells

chip antibody

CHD4 (Abcam, ab70469)

cell type

ZFP296 CRISPR KO clone 7 R1 ESCs

culture conditions

Serum/LIF +2i

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Libraries were prepared for Illumina sequencing using 5 ng of ChIP material with a Kapa Hyper Prep Kit (Kapa Biosystems), according to the manufacturer's instructions

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

14335702

Reads aligned (%)

88.1

Duplicates removed (%)

9.8

Number of peaks

1622 (qval < 1E-05)

mm9

Number of total reads

14335702

Reads aligned (%)

88.0

Duplicates removed (%)

10.8

Number of peaks

1623 (qval < 1E-05)