SRX4403321
GSM3290464: ESC WT spikein H3K27ac; Mus musculus; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
Embryonic stem cells
tissue
Embryonic stem cells
chip antibody
H3K27ac (Diagenode, A1723-0041d)
cell type
WT R1 ESCs
culture conditions
Serum/LIF +2i

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were prepared for Illumina sequencing using 5 ng of ChIP material with a Kapa Hyper Prep Kit (Kapa Biosystems), according to the manufacturer's instructions

Sequencing Platform

instrument_model
NextSeq 500

Where can I get the processing logs?

Read processing pipeline log

mm10

Number of total reads
29694031
Reads aligned (%)
93.1
Duplicates removed (%)
8.7
Number of peaks
35604 (qval < 1E-05)

mm9

Number of total reads
29694031
Reads aligned (%)
93.0
Duplicates removed (%)
8.9
Number of peaks
35610 (qval < 1E-05)

Base call quality data from DBCLS SRA