Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Mbd3

Cell type

Cell type Class
Pluripotent stem cell
Cell type
mESC derived neural cells
NA
NA

Attributes by original data submitter

Sample

source_name
Neural progenitor cells
tissue
Neural progenitor cells
chip antibody
MBD3 (Bethyl, A302-528A)
cell type
WT R1 ESC-derived NPCs
culture conditions
NPC medium

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Libraries were prepared for Illumina sequencing using 5 ng of ChIP material with a Kapa Hyper Prep Kit (Kapa Biosystems), according to the manufacturer's instructions

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
50308394
Reads aligned (%)
95.9
Duplicates removed (%)
35.2
Number of peaks
2977 (qval < 1E-05)

mm9

Number of total reads
50308394
Reads aligned (%)
95.7
Duplicates removed (%)
35.2
Number of peaks
2925 (qval < 1E-05)

Base call quality data from DBCLS SRA