Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Pluripotent stem cell

Cell type

iPS cells

NA

NA

Sample

source_name

Angelman Syndrome iPSC, rep 1

cell type

Angelman Syndrome iPSC

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq extraction performed with Millipore EZ-Magna ChIP G – Chromatin Immunoprecipitation Kit (17-409) from 6-10 million cells, 4C-seq extraction from ~2 million formaldehyde-fixed cells according to Cotney JL & Noonan JP ((2015) Cold Spring Harb Protoc 2015(4):419.) ChIP-seq library preparation performed by the Yale Stem Cell Center Genomics Core; 4C-seq library prepared according to van de Werken HJ, et al. ((2012) Nat Methods 9(10):969-972.) using NlaIII for the first restriction digestion and DpnII for the second restriction digestion.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

46932581

Reads aligned (%)

78.2

Duplicates removed (%)

3.9

Number of peaks

832 (qval < 1E-05)

hg19

Number of total reads

46932581

Reads aligned (%)

77.6

Duplicates removed (%)

4.5

Number of peaks

908 (qval < 1E-05)